Document 0779
 DOCN  M94B0779
 TI    Subtypes of Epstein-Barr virus in HIV-1-positive and HIV-1-negative
       patients with Hodgkin's disease (Meeting abstract).
 DT    9412
 AU    De Re V; De Vita S; Marzotto A; Gloghini A; Carbone A; Uccini S; Scarpa
       A; and Boiocchi M; Div. of Experimental Oncology, Aviano, Italy
 SO    EACR-12: 12th Biennial Meeting of the European Association for Cancer
       Research. April 4-7, 1993, Brussels, Belgium, 1993.. Unique Identifier :
       AIDSLINE ICDB/94697510
 AB    Considerable evidence suggests that EBV has a role in the pathogenesis
       of Hodgkin's disease (HD). In immunocompetent hosts type 2 EBV is
       considered to be a much less potent transformer of lymphocytes than type
       1 virus strain. However, type 2 EBV may be involved in the pathogenesis
       of non-Hodgkin's lymphomas arising in immunocompromised patients (ie,
       those with HIV-1 or malarial infection). To determine whether type 2 EBV
       may also play a role in similar patients developing HD, we characterized
       EBV subtypes, using the PCR technology, in 36 tumor samples (previously
       demonstrated to be EBV-positive by amplification of the IR3 genome
       sequence common to both EBV strains) from 10 HIV-1-positive patients as
       well as from a control population of 26 HIV-1-negative patients. Type 2
       EBV was detected in 5 samples from HIV-1-positive patients, and in only
       1 sample from the HIV-1-negative group. DNA of one HIV-1-positive
       patient and DNA of one HIV-1-negative patient was positive for both EBV
       type 1 and EBV type 2. DNA sequence analysis of EBNA-2 region indicated
       the concomitant presence of both EBV type 1 and EBV type 2 expansions
       and excluded a possible 'hybrid-recombinant' in these two tumor samples.
       A biclonal EBV tumor infection was also detected by Southern blot in two
       additional HIV-1-negative cases, both clones belonging, this time, to
       type 1 virus strain. In six EBV-positive cases (all from HIV-1-negative
       patients), characterization of the virus could not be performed
       presumably on account of deletion including EBNA-2 region. Our results
       indicate that in HIV-1-infected patients type 2 EBV presents a
       transforming ability that is roughly similar to that shown by type 1
       virus strain also in patients developing HD.
 DE    Acquired Immunodeficiency Syndrome/COMPLICATIONS/*MICROBIOLOGY
       Antigens, Viral/GENETICS  DNA-Binding Proteins/GENETICS  Gene
       Amplification  Genome, Viral  HIV Seropositivity/GENETICS/*MICROBIOLOGY
       *Herpesvirus 4, Human/GENETICS  Hodgkin's
       Disease/COMPLICATIONS/GENETICS/*MICROBIOLOGY  Human  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).